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2021 |
Development of a real-time recombinase-aided amplification (RT-RAA) molecular diagnosis assay for sensitive and rapid detection of Toxoplasma gondii |
Zhao-Hua Wang,Wei Zhang,Xiu-Zhong Zhang,Xin-Ran Yao,Wei Huang,Hong Jia,Xiao-Lei Liu,Shao-Hua Hou,Xiao-Jia Wang |
Veterinary parasitology |
34384956 |
10.1016/j.vetpar.2021.109489 |
Development of a real-time recombinase-aided amplification (RT-RAA) molecular diagnosis assay for sensitive and rapid detection of Toxoplasma gondii
Author(s):
Zhao-Hua Wang,Wei Zhang,Xiu-Zhong Zhang,Xin-Ran Yao,Wei Huang,Hong Jia,Xiao-Lei Liu,Shao-Hua Hou,Xiao-Jia Wang
Journal:
Veterinary parasitology
Year:
2021
Abstract:
Toxoplasma gondii, a protozoan intracellular parasite, is present in a wide range of hosts, including virtually all species of warm-blooded vertebrates. Toxoplasmosis spreads to humans through a variety of pathways, including contaminated food or water, and close contact with various types of domestic animals. It poses a severe threat to human health, and contributes to important economic losses, not only in cost-of-illness but also in surveillance programs. It is thus necessary to develop a rapid point-of-care field diagnostic technology to control or prevent pathogen transmission to economically important livestock animals, domestic animals, and human beings. In this study, we develop a real-time isothermal amplification method capable of detecting the T. gondii genome in swine and feline blood samples. This method can detect toxoplasma genome with a lowest detection limit of 102 copies of per reaction under optimal reaction conditions of 36 °C for 25 min. The assay displayed advantages in sensitivity and specificity in comparison to traditional real-time PCR, and can be performed in a portable instrument.
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