RPB0545

Pathogen Description

Target Pathogen Pathogen Name NCBI Taxonomy ID Order Family Genus Species Pathogen type
Tick-Borne Encephalitis Virus Tick-borne encephalitis virus,TBEV,FSME virus,Tick born encephalitis virus,tick-borne encephalitis virus TBE virus,tick-borne encephalitis virus TBEV 11084 Amarillovirales Flaviviridae Orthoflavivirus Orthoflavivirus encephalitidis Viruses

Primer Description

Primer Name Sequence(5'-3') Length(bp) Primer Final Concentration(μM） GC Content（%） Predicted Melting Temperature(℃） Molecular Weight(g/moles) Positions in GenBank accession number
TBEV-F3 GAAGCATGCTTCCGGGAGGAGGGAAGAGAG 30 \ 60 68.04 9435.18 \
TBEV-R1 TCCGAGTCACACGTCACCTCCTTGTCAGACT 31 \ 54.84 67.41 9367.13 \
TBEV-P AGGGGGGGCGGTTCTTGTTCTCCCTGAGCCFCHAQCACCCAGACACAGAT-[3’-block] 48 \ 61.1 78.12 14743.57 \

Gene Description

Target Gene GenBank ID
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Assay Description

Application Assay Primer Designing Software Reaction Time(min) Assay Temperature(℃) Readout System(s) Limit of Detection(LoD) Sensitivity(%) Specificity(%)
Our RT-RPA assay may be useful for detection of TBEV infection under field conditions. RT-RPA \ 20 min \ \ 10 copies/reaction \ \

Publication Description

Year of Publication Title Author(s) Journal PMID DOI
2019 Reverse Transcription Recombinase Polymerase Amplification Assays for Rapid Detection of Tick-Borne Encephalitis Virus Infection Jia Jia,Yuchang Li,Xiaoyan Wu,Sen Zhang,Yi Hu,Jing Li,Tao Jiang,Xiaoping Kang Virologica Sinica 30941699 10.1007/s12250-019-00105-4

Reverse Transcription Recombinase Polymerase Amplification Assays for Rapid Detection of Tick-Borne Encephalitis Virus Infection

Author(s):

Jia Jia,Yuchang Li,Xiaoyan Wu,Sen Zhang,Yi Hu,Jing Li,Tao Jiang,Xiaoping Kang

Journal:

Virologica Sinica

Year:

2019

Abstract:

No abstract available.