RPB0292

Pathogen Description

Target Pathogen Pathogen Name NCBI Taxonomy ID Order Family Genus Species Pathogen type
SARS-CoV-2 SARS-CoV-2, 2019-nCoV, COVID-19, COVID-19 virus, SARS2, Wuhan coronavirus, Human coronavirus 2019, COVID19, HCoV-19, SARS-2, SARS-CoV4 2697049 Nidovirales Coronaviridae Betacoronavirus Severe acute respiratory syndrome-related coronavirus virus

Primer Description

Primer Name Sequence(5'-3') Length(bp) Primer Final Concentration(μM） GC Content（%） Predicted Melting Temperature(℃） Molecular Weight(g/moles) Positions in GenBank accession number
F 5′ FAM-AGTTCAAGAAATTCAACTCCAGGCAGCAGTA 31 1 μM 41.94 61.2 9521.28 \
R 5′ Biotin-GCCTCAGCAGCAGATTTCTTAGTGACAGTTT 31 1 μM 45.16 62.07 9501.23 \

Gene Description

Target Gene GenBank ID
N gene \

Assay Description

Application Assay Primer Designing Software Reaction Time(min) Assay Temperature(℃) Readout System(s) Limit of Detection(LoD) Sensitivity(%) Specificity(%)
a compact, portable, rapid RT-RPA kit for target-specific viral RNA amplification and detection in one platform. RT-RPA-LFA \ 20 min 39°C LFA 10 copies μL-1 \ \

Publication Description

Year of Publication Title Author(s) Journal PMID DOI
2025 A portable, rapid isothermal amplification kit enabling naked eye detection of SARS-CoV-2 RNAs Ji-Ho Park,Kihyeun Kim,Bobin Lee,Hyungjun Jang,Min-Gon Kim Talanta 39657523 10.1016/j.talanta.2024.127327

A portable, rapid isothermal amplification kit enabling naked eye detection of SARS-CoV-2 RNAs

Author(s):

Ji-Ho Park,Kihyeun Kim,Bobin Lee,Hyungjun Jang,Min-Gon Kim

Journal:

Talanta

Year:

2025

Abstract:

Since the coronavirus disease 2019 (COVID-19) pandemic, isothermal amplification techniques have attracted attention due to their higher sensitivity and specificity, compared with immunoassays, and their potential application for point-of-care testing (POCT). A requirement of isothermal amplification-based POCT kits is the inclusion of a heating source with an electrical power supply. We developed an amplification-based rapid kit, which is a portable and naked eye-detectable reverse transcriptase (RT)-recombinase polymerase amplification (RPA) kit. The rapid RT-RPA kit consists of a flow-controllable paper chip, a nickel-chromium (NiCr)-based Joule-heating thin film, and a small-sized portable battery. We found that the Joule-heating thin film, powered by a lithium-ion battery (7.5 g, 20 mm × 35 mm size), was able to maintain the required temperature for the RPA reaction. After the RPA reaction, which takes approximately 20 min, the flow-controllable paper chip automatically enabled visualization of the amplicon by time-delayed release of gold nanoparticle-based optical probes. Using this system, we successfully detected severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) at levels as low as 10 copies μL-1, within 30 min.