RPB0233

Pathogen Description

Target Pathogen Pathogen Name NCBI Taxonomy ID Order Family Genus Species Pathogen type
SARS-CoV-2 SARS-CoV-2, 2019-nCoV, COVID-19, COVID-19 virus, SARS2, Wuhan coronavirus, Human coronavirus 2019, COVID19, HCoV-19, SARS-2, SARS-CoV4 2697049 Nidovirales Coronaviridae Betacoronavirus Severe acute respiratory syndrome-related coronavirus virus

Primer Description

Primer Name Sequence(5'-3') Length(bp) Primer Final Concentration(μM） GC Content（%） Predicted Melting Temperature(℃） Molecular Weight(g/moles) Positions in GenBank accession number
RPA-SARS-CoV-2-F GATTGAACCAGCTTGAGAGCAAAAT 25 \ 40 55.63 7723.11 \
RPA-SARS-CoV-2-R ATTTCCTTGGGTTTGTTCTGGACCACGT 28 \ 46.43 62.41 8567.6 \

Gene Description

Target Gene GenBank ID
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Assay Description

Application Assay Primer Designing Software Reaction Time(min) Assay Temperature(℃) Readout System(s) Limit of Detection(LoD) Sensitivity(%) Specificity(%)
a multichannel, rapid,Specially and sensitive electrochemical sensing platform for the detection of H1N1, H3N2 and SARS-CoV-2. RPA-CRISPR approach with a microfluidic chip primer 5.0 30 min 39℃ CRISPR Cas12a system 5 copies \ \

Publication Description

Year of Publication Title Author(s) Journal PMID DOI
2024 Detection and identification of SARS-CoV-2 and influenza a based on microfluidic technology Yujie Liu,Guanliu Yu,Hongkun Liang,Wenbo Sun,Lulu Zhang,Michael G Mauk,Hua Li,Lei Chen Analytical Methods 38919038 10.1039/d4ay00847b

Detection and identification of SARS-CoV-2 and influenza a based on microfluidic technology

Author(s):

Yujie Liu,Guanliu Yu,Hongkun Liang,Wenbo Sun,Lulu Zhang,Michael G Mauk,Hua Li,Lei Chen

Journal:

Analytical Methods

Year:

2024

Abstract:

As of now, the global COVID-19 pandemic caused by SARS-CoV-2, which began in 2019, has been effectively controlled. However, the symptoms of influenza A virus infection were similar to those of SARS-CoV-2 infection, but they required different treatment approaches. To make the detection more accurate and the treatment more targeted. We developed a system that integrates RPA and CRISPR assays, allowing for the rapid, highly specific, and sensitive detection and differentiation of SARS-CoV-2, H1N1, and H3N2. Under isothermal amplification conditions, the RPA-CRISPR Cas12a detection system achieved a detection limit as low as 5 copies per μL, demonstrating excellent specificity. The measurement time was approximately 30 minutes. The RPA-CRISPR Cas12a detection system combined with the microfluidic chip we designed to simultaneously detect three viruses, providing a potential solution for efficient and reliable diagnosis.