RPB0205

Pathogen Description

Target Pathogen Pathogen Name NCBI Taxonomy ID Order Family Genus Species Pathogen type
SARS-CoV-2 SARS-CoV-2, 2019-nCoV, COVID-19, COVID-19 virus, SARS2, Wuhan coronavirus, Human coronavirus 2019, COVID19, HCoV-19, SARS-2, SARS-CoV4 2697049 Nidovirales Coronaviridae Betacoronavirus Severe acute respiratory syndrome-related coronavirus virus

Primer Description

Primer Name Sequence(5'-3') Length(bp) Primer Final Concentration(μM） GC Content（%） Predicted Melting Temperature(℃） Molecular Weight(g/moles) Positions in GenBank accession number
F FITC-AGTGCGTTGTTCGTTCT 17 0.2 47.1 51.26 5198.43 \
R CATTCTGCTTCCAAGT-/C12/-TTTGATCGCGCCCCACTG 34 0.2 54.3 68.75 10584.9 \

Gene Description

Target Gene GenBank ID
N-gene \

Assay Description

Application Assay Primer Designing Software Reaction Time(min) Assay Temperature(℃) Readout System(s) Limit of Detection(LoD) Sensitivity(%) Specificity(%)
\ duplex RT-RPA-LFS DNAStarLasergenev7.1 25 39 flow strip \ \ \

Publication Description

Year of Publication Title Author(s) Journal PMID DOI
2022 Rapid and simultaneous visual screening of SARS-CoV-2 and influenza virufses with customized isothermal amplification integrated lateral flow strip Yong Sun , Panzhu Qin , Jun He , Weiwei Li , Yonglin Shi , Jianguo Xu , Qian Wu , Qingqing Chen , Weidong Li , Xinxin Wang , Guodong Liu , Wei Chen Biosensors and Bioelectronics 34775255 10.1016/j.bios.2021.113771

Rapid and simultaneous visual screening of SARS-CoV-2 and influenza virufses with customized isothermal amplification integrated lateral flow strip

Author(s):

Yong Sun , Panzhu Qin , Jun He , Weiwei Li , Yonglin Shi , Jianguo Xu , Qian Wu , Qingqing Chen , Weidong Li , Xinxin Wang , Guodong Liu , Wei Chen

Journal:

Biosensors and Bioelectronics

Year:

2022

Abstract:

Due to the similar clinical symptoms of influenza (Flu) and coronavirus disease 2019 (COVID-19), there is a looming infection threat of concurrent Flu viruses and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). In this work, we introduce a customized isothermal amplification integrated lateral flow strip (LFS) that is capable performing duplex reverse transcription-recombinase polymerase amplification (RT-RPA) and colorimetric LFS in a sequential manner. With customized amplification primer sets targeted to SARS-CoV-2 (opening reading frame 1a/b and nucleoprotein genes) and Flu viruses (Flu A and Flu B), the platform allows the rapid and simultaneous visual screening of SARS-CoV-2 and Flu viruses (Flu A and Flu B) without cross reactivity, false positives, and false negatives. Moreover, it maximally eases the detection, reduces the detection time (1 h), and improves the assay performance to detect as low as 10 copies of the viral RNA. Its clinical application is powerfully demonstrated with 100% accuracy for evaluating 15 SARS-CoV-2-positive clinical samples, 10 Flu viruses-positive clinical samples, and 5 negative clinical samples, which were pre-confirmed by standard qRT-PCR. We envision this portable device can meet the increasing need of online monitoring the serious infectious diseases that substantially affects health care systems worldwide.