RPB0128

Pathogen Description

Target Pathogen Pathogen Name NCBI Taxonomy ID Order Family Genus Species Pathogen type
RSV A/B Respiratory syncytial virus, Respiratory syncytial virus RS, respiratory syncytial virus RS virus, Respiratory syncytial virus RSV 12814 Mo\gavirales Pneumoviridae Orthopneumovirus Bovine orthopneumovirus Virus

Primer Description

Primer Name Sequence(5'-3') Length(bp) Primer Final Concentration(μM） GC Content（%） Predicted Melting Temperature(℃） Molecular Weight(g/moles) Positions in GenBank accession number
forward primer FITC-TCCYARTTGTATAGCATTCATAGGTGAAGGAG 32 0.42 40.6 58.72 9903 \
reverse primer Biotin-TTGCATCTGTAGCAGGAATGGTYAAATTYTCA 32 0.42 37.5 59.5 9853.47 \

Gene Description

Target Gene GenBank ID
conserved L gene \

Assay Description

Application Assay Primer Designing Software Reaction Time(min) Assay Temperature(℃) Readout System(s) Limit of Detection(LoD) Sensitivity(%) Specificity(%)
detection of respiratory syncytial virus RT-RPA-LFD \ 30 37 LFD 10 copies of a given RNA molecule 1 1

Publication Description

Year of Publication Title Author(s) Journal PMID DOI
2020 Utilization of recombinase polymerase amplification method combined with lateral flow dipstick for visual detection of respiratory syncytial virus. Xu, Yu-Zhong; Fang, Du-Zhi; Chen, Fang-Fang; Zhao, Qin-Fei; Cai, Chao-Ming; Cheng, Ming-Gang; MOL CELL PROBE 31654732 10.1016/j.mcp.2019.101473

Utilization of recombinase polymerase amplification method combined with lateral flow dipstick for visual detection of respiratory syncytial virus.

Author(s):

Xu, Yu-Zhong; Fang, Du-Zhi; Chen, Fang-Fang; Zhao, Qin-Fei; Cai, Chao-Ming; Cheng, Ming-Gang;

Journal:

MOL CELL PROBE

Year:

2020

Abstract:

Respiratory syncytial virus (RSV) is a major causative agent of respiratory tract infection necessitating hospitalization in children. A rapid diagnostic method would facilitate early detection of RSV infection and timely implementation of special treatment. Here, a reverse transcription recombinase polymerase amplification (RT-RPA) assay combined with lateral flow dipstick (LFD) was evaluated for rapid visual detection of RSV. The primers were designed to target the conserved L gene. The RT-RPA-LFD assay could simultaneously detect RSV subtype A and B with the same detection limit of 10 copies of a given RNA molecule. Moreover, the assay showed no cross-reactivity with other common human pathogens. The performance of the RT-RPA-LFD assay was evaluated by testing 136 nasopharyngeal aspirates (NPAs). The agreement of the detection results between RT-RPA-LFD and qRT-PCR was 100% (34 positive and 102 negative cases). In summary, the developed RT-RPA-LFD assay had good performance in detecting RSV in clinical specimens, thus providing a novel alternative solution for the detection of RSV under field conditions.