RPB0087

Pathogen Description

Target Pathogen Pathogen Name NCBI Taxonomy ID Order Family Genus Species Pathogen type
EV-A71 Enterovirus 71, Enterovirus EV-A71, Human enterovirus 71, Human enterovirus A71, Human enterovirus type 71, enterovirus type 71 39054 Picornavirales Picornaviridae Enterovirus Enterovirus A Virus

Primer Description

Primer Name Sequence(5'-3') Length(bp) Primer Final Concentration(μM） GC Content（%） Predicted Melting Temperature(℃） Molecular Weight(g/moles) Positions in GenBank accession number
EV71-F1 TGCCATTCATGTCACCTGCGAGTGCTTATC 30 0.42 50 64.38 9123.97 \
EV71-R1 CCTGACGTGCTTCATTCTCATGTAAATCCTAA 32 0.42 40.6 59.51 9709.38 \
EV71-TPR1 CCCACAGTCCGCACTGAGAACGTGCCCA-[FAM-dT]-C-[THF][BHQ1-dT]-GTTATTAGGACATGC-[C3-spacer] 44 0.12 56.8 73.09 13446.77 \

Gene Description

Target Gene GenBank ID
VP1 gene GQ279370.1

Assay Description

Application Assay Primer Designing Software Reaction Time(min) Assay Temperature(℃) Readout System(s) Limit of Detection(LoD) Sensitivity(%) Specificity(%)
detection of human enterovirus 71 RT-RPA BLAST tools of the NCBI 20 40 exo probe 3.767 log10 genomic copies (LGC)/reaction 0.895 1

Publication Description

Year of Publication Title Author(s) Journal PMID DOI
2018 Development and evaluation of a rapid recombinase polymerase amplification assay for the detection of human enterovirus 71 Dan Yin,Yanan Zhu,Kaifeng Wang,Jing Wang,Xiru Zhang,Min Han,Yaqing He,Qing Chen,Guifang Hu Archives of virology 29767300 10.1007/s00705-018-3859-x

Development and evaluation of a rapid recombinase polymerase amplification assay for the detection of human enterovirus 71

Author(s):

Dan Yin,Yanan Zhu,Kaifeng Wang,Jing Wang,Xiru Zhang,Min Han,Yaqing He,Qing Chen,Guifang Hu

Journal:

Archives of virology

Year:

2018

Abstract:

Enterovirus 71 (EV71) is one of the most common pathogens of hand, foot, and mouth disease (HFMD). A rapid reverse transcription recombinase polymerase amplification (RT-RPA) assay was established to detect EV71 subgenotype C4 (EV71-C4). The 95% detection limit of the RT-RPA was 3.767 log10 genomic copies (LGC)/reaction. The specificity was 100%. In a clinical sample evaluation, this approach demonstrated sufficient clinical performance when compared with a commercial RT-qPCR diagnostic kit. Thus, the RT-RPA assay may be a promising alternative for the detection of EV71-C4.