RPB0032

Pathogen Description

Target Pathogen Pathogen Name NCBI Taxonomy ID Order Family Genus Species Pathogen type
SARS-CoV-2 SARS-CoV-2, 2019-nCoV, COVID-19, COVID-19 virus, SARS2, Wuhan coronavirus, Human coronavirus 2019, COVID19, HCoV-19, SARS-2, SARS-CoV4 2697049 Nidovirales Coronaviridae Betacoronavirus Severe acute respiratory syndrome-related coronavirus virus

Primer Description

Primer Name Sequence(5'-3') Length(bp) Primer Final Concentration(μM） GC Content（%） Predicted Melting Temperature(℃） Molecular Weight(g/moles) Positions in GenBank accession number
F CTTGAAATTCCACGTAGGAATGTGGCAACTTTAC 34 0.32 41.2 60.71 10440.85 \
R GTATGCCAGGTATGTCAACACATAAACCTTCAG 33 0.32 42.4 60.26 10105.65 \

Gene Description

Target Gene GenBank ID
ORF1ab NC-045512.2

Assay Description

Application Assay Primer Designing Software Reaction Time(min) Assay Temperature(℃) Readout System(s) Limit of Detection(LoD) Sensitivity(%) Specificity(%)
as POCT candidates for convenient screening of infectious viruses such as SARS-CoV-2 FeS2-RPA \ 20 39 interpreted with the naked eye(strips) 200 copies/mL \ \

Publication Description

Year of Publication Title Author(s) Journal PMID DOI
2022 Nanozyme-strip for rapid and ultrasensitive nucleic acid detection of SARS-CoV-2 Xiangqin Meng, Sijia Zou, Dandan Li , Jiuyang He, Ling Fang, Haojue Wang, Xiyun Yan, Demin Duan, Lizeng Gao Biosensors & Bioelectronics 36155953 10.1016/j.bios.2022.114739

Nanozyme-strip for rapid and ultrasensitive nucleic acid detection of SARS-CoV-2

Author(s):

Xiangqin Meng, Sijia Zou, Dandan Li , Jiuyang He, Ling Fang, Haojue Wang, Xiyun Yan, Demin Duan, Lizeng Gao

Journal:

Biosensors & Bioelectronics

Year:

2022

Abstract:

The coronavirus disease 2019 (COVID-19) pandemic has created a huge demand for sensitive and rapid detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The current gold standard for SARS-CoV-2 detection is reverse transcription-polymerase chain reaction (RT-PCR)-based nucleic acid amplification. However, RT-PCR is time consuming and requires specialists and large instruments that are unattainable for point-of-care testing (POCT). To develop POCT for SARS-CoV-2, we combined recombinase polymerase amplification (RPA) and FeS2 nanozyme strips to achieve facile nucleic acid amplification and subsequent colorimetric signal enhancement based on the high peroxidase-like activity of the FeS2 nanozymes. This method showed a nucleic acid limit of detection (LOD) for SARS-CoV-2 of 200 copies/mL, close to that of RT-PCR. The unique catalytic properties of the FeS2 nanozymes enabled the nanozyme-strip to amplify colorimetric signals via the nontoxic 3,3',5,5'-tetramethylbenzidine (TMB) substrate. Importantly, the detection of clinical samples of human papilloma virus type 16 (HPV-16) showed 100% agreement with previous RT-PCR results, highlighting the versatility and reliability of this method. Our findings suggest that nanozyme-based nucleic acid detection has great potential in the development of POCT diagnosis for COVID-19 and other viral infections.